PATHOTYPING AND ANTIMICROBIAL RESISTANCE PATTERN OF Escherichia coli ISOLATES FROM RAW MILK AND DAIRY ENVIRONMENT
Loading...
Date
2022
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Escherichia coli is an common food pathogen with widespread
distribution. Hence, the present study has been contemplated to ascertain the
presence of E.coli in bovine milk and dairy farm environmental interface and to
determine the pathotype by molecular assay. Further, antibiotic resistance patterns
of the E.coli isolates were determined by both phenotypic and genotypic methods.
A total of 230 samples comprising of 116 milk, 54 dung, 30 swab from
dairy equipments, 15 water and 15 air samples were collected from both organised
and unorganised bovine farms in and around Chennai City, Tamil Nadu, India
were screened for the presence of E.coli by conventional method viz., cultural and
biochemical tests and the presumptive colonies were further confirmed by PCR
targeting uspA gene. The distribution of positive isolates obtained from various
samples viz. raw milk samples (25/116), dung (15/54), water (8/15), air (4/15) and
swab (3/30) with overall occurrence of E.coli in the present study was 23.91
percent (55/230). All the presumptive isolates were biochemically characterized
and were subjected to PCR assay. Results of our study have clearly indicated 100
percent correlation between biochemical test and PCR assay.