EFFECT OF VITAMIN C AND D AGAINST RESISTANT BACTERIA ISOLATED FROM POULTRY ORIGIN
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Date
2022
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Abstract
The present study was conducted to obtain the idea about resistance pattern in E. coli from broiler chickens by qualitative and quantitative methods and the isolates were assessed for presence of resistant genes through PCR. Then the effect of vitamin C and vitamin D3 was studied against E. coli isolates when used in combination with resistant antimicrobial agents.
The liver swabs were collected aseptically from birds suspected to be died of colibacillosis and transported to laboratory under refrigerator condition. Then E. coli was isolated from 60% of samples through phenotypic and genotypic
characterization by targeting Adk gene after pre-enrichment of swabs with nutrient broth and selective isolation using Macconkey and EMB agar.
Antimicrobial sensitivity screening of confirmed E. coli isolates were done by Kirby-Bauer disc diffusion technique for commonly used antimicrobial agents in broilers. In this enrofloxacin and tetracycline showed maximum resistance of 83.33 % and 69.44 % respectively. Among this 8.33% of samples were found to ESBL producing phenotypically.