ASSESSMENT OF ANTIMICROBIAL RESIDUES BY MICROBIAL ASSAY AND CHROMATOGRAPHIC TECHNIQUES IN MILK AND MILK POWDER
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Date
2021
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Abstract
An assessment of antimicrobial residues in dairy milk and milk powder was conducted to ensure the safety of the milk intended for human consumption. A total number of 300 milk samples and 200 samples of milk powder were collected for antimicrobial residue assessment. Out of 300 milk samples, 100 cow milk samples and 100 buffalo milk samples were collected from organised dairy farms in and around Chennai, Tamil Nadu and the remaining 100 homogenised milk samples were collected from the commercial outlets in and around Chennai. Out of 200 milk powder samples, 100 skimmed milk powder samples and 100 whole milk powder samples from different manufacturers and different batches were collected from the commercial outlets in and around Chennai for analysis of antimicrobial residues.
The collected milk and milk powder samples were analyzed for six classes of antibiotic groups namely Aminoglycosides, Macrolides, Quinolones, Tetracyclines, Betalactams and Polypeptides. Screening of 300 milk samples and 200 milk powder samples for antimicrobial residues was conducted by using microbiological tube test method with their suitable indicator organisms such as Bacillus subtilis, Escherichia coli, Geobacillus stearothermophilus.