DEVELOPMENT OF RECOMBINANT FLAGELLAR PROTEIN BASED IMMUNODIAGNOSTIC ASSAY FOR DETECTION OF Campylobacter jejuni
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Date
2021
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Abstract
Campylobacter spp. is a zoonotic pathogen and most reported cause of human gastroenteritis worldwide. Campylobacter jejuni is a commensal organism of the intestinal tract of poultry and other agriculturally important animals. The major transmission routes of Campylobacteriosis in human are consumption of contaminated or undercooked meat, raw milk or improper pasteurized milk. This study was undertaken to investigate the incidence of Campylobacter jejuni in milk, meat and faecal samples. A total of 143 samples comprising mastitis milk (20), raw milk (52), faecal swab of dog (19) suffer from diarrhoea, raw chicken meat (19), meat sample from sheep (5) and goat (3), beef sample (25) were collected aseptically. The samples were studied for cultural, biochemical and molecular examinations. Out of 143 samples 123 samples showed growth on modified blood free charcoal cefoperazone deoxycholate (mCCDA) agar media and 75 (60.97 %) isolates were identified as Campylobacter based on their morphology, Gram`s staining and motility. The isolated bacteria were characterized as C. jejuni based on biochemical tests viz. oxidase test, catalase test, nitrate reduction test, triple sugar iron test (TSI), hippurate hydrolysis and ninhydrin test specific for identification of C. jejuni and 47 (38.21 %) isolates were confirmed as C. jejuni. Based on triple sugar iron test 31 isolates were negative and catagorised as C. jejuni biotype 1 while 16 isolates were positive as biotype 2.