MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF KIWIFRUIT (Actinidia spp.) GERMPLASM
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Date
2023-11-09
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UHF,NAUNI
Abstract
ABSTRACT
The present investigation entitled "Characterization of kiwifruit (Actinidia spp.)
germplasm through morphological and molecular analysis," aimed to assess the genetic
diversity of 40 genotypes belonging to three Actinidia species: Actinidia arguta (10
genotypes), Actinidia chinensis (24 genotypes) and Actinidia deliciosa (6 genotypes) in the
experimental field and Molecular Breeding Laboratory of Department of Fruit Science, Dr
YS Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh. There
was significant variation in the quantitative characteristics studied, including shoot thickness,
leaf blade length and width, leaf area, petiole length, number of flowers per shoot and
flowering duration. Morphological characteristics were recorded as per the National Test
guidelines for DUS by UPOV. The variation in qualitative parameters namely, vine sex
forms, stem: texture of bark, leaf blade shape, shape of apex and type of inflorescence among
three species were also observed. The diverse genotypes based on the morphological
characterization were, A-3 (♀), A-4 (♀), A-9 (♂), A-6 (♀) and A-10 (⚥) in Actinidia arguta,
while, PS-3 (♀), PS-4 (♀), PS-5 (♀), PS-7(♂), PS-8 (♂), PS-9 (♀) and PS-19 (♂) in
Actinidia chinensis and Allison (♀), Bruno (♀), Monty (♀) and Tomuri (♂) in Actinidia
deliciosa. Out of total 21 simple sequence repeats (SSRs) primers, seven primers UDK96-
009, UDK96-016, UDK96-030, UDK96-033, UDK 96-026, UDK96-035 and UDK 96-034
showed polymorphism (31.98 %) with PIC value 0.210. The distinctive genotypes on the
basis of above primers used were A-2 (♀), A-4 (♀), A-5 (♂), A-6 (♀), A-9 (♂) and A-10 (⚥)
in Actinidia arguta, while, in Actinidia chinensis, PS-1 (♀), PS-8 (♂), PS-10 (♂), PS-12 (♂),
PS-13(♂), PS-14 (♂), PS-15 (♂), PS-17 (♂) and PS-24 (♂) and in Actinidia deliciosa,
Allison (♀), Bruno (♀), Monty (♀) and Tomuri (♂). The observed genetic richness of
superior genotypes and the information generated during the study can be used as selection
indices for improvement in kiwifruit breeding programs.