ENHANCING EFFICACY OF CAPSULE BASED FORMULATIONS AND MOLECULAR CHARACTERIZATION OF LOCAL ISOLATES OF Lecanicillium lecanii (Zimm.) Zare and Gams.
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Date
2021-02
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University of Horticultural Sciences, Bagalkot. (College of Horticulture, Bagalkot).
Abstract
The research entitled “Enhancing efficacy of capsule based formulations and
molecular characterization of local isolates of Lecanicillium lecanii (Zimm.) Zare and
Gams” was carried out at Department of Entomology, College of Horticulture Bagalkot,
UHS, Bagalkot during 2019-2020. The principle objectives of study were, 1. Enhancing
the efficacy of capsule formulations of local isolates of L. lecanii. 2. Molecular
characterization of local isolates of L. lecanii. The capsule formulations of L. lecanii was
enhanced their efficacy against Myzus persicae by incorporating various additives.
Chitosan added in the equal ratio to spore powder and talc in (1:1:1) resulted better
efficacy in both laboratory and pot culture experiments, followed by spore powder and talc
(1:1) and spore powder +talc + dextrin (1:1:1).
Among the tested combinations, capsule containing spore powder + talc + chitosan
(1:1:1) proved better efficacy against aphids when stored at refrigerated condition after 90
days of storage period followed by mud pot filled with sand and room temperature. The
same formulation was tested after 90 days of storage period. The efficacy of formulations
were tested against pot cultured cabbage seedlings against M. persicae under pot culture
experiment and proved highest mortality of aphids when compared with commercial
formulations of L. lecanii and NSKE at 5%. However chlorantraniliprole showed
significantly highest mortality of aphids found to be superior over all the treatments.
Molecular characterization of local isolates of L. lecanii was done using universal
primers ITS-1 and ITS-4 as forward and backward primers, respectively. DNA sequencing
resulted that, LL-1 groups with uncultured fungus group and LL-2 and LL-3 joined with
other fungal endophyte group. Morphologically these isolates identified as L. lecanii by
Agharkar research institute, Pune, whereas molecular study indicated that, these isolates
belongs to other fungal groups. Hence, reconfirmation work to be carried out.