EXPRESSION PROFILING OF CANDIDATE EMBRYOTROPHIC GENES OF BUFFALO OVIDUCT DURING DIFFERENT STAGES OF OESTROUS CYCLE
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Date
2022
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Abstract
The aim of the present study was to
identify the gene expression profile of three
candidate genes namely heat shock protein 70,
oviductal glycoprotein and osteopontin in buffalo
oviduct during different stages of the oestrous
cycle. Slaughter house derived buffalo oviducts
were categorized as belonging to early, mid and
late phase of the oestrous cycle based on the
morphological appearance of CL and progesterone
concentration. The relative abundance of each of
the candidate gene transcript was quantified by
real time RT-PCR. Transcripts of all the three
candidate genes were found to be expressed in
OEC throughout the oestrous cycle. The results of
the present study demonstrate that gene expression
in the buffalo oviduct is clearly regulated during
the oestrous cycle, as these candidate genes play
a crucial role in several reproductive processes.
It was found that Hsp 70 gene was significantly
upregulated in OEC belonging to the early and
late phases when compared to mid phases of the
cycle. The expression of OVGP mRNA was found
to be maximum during the early phase and it
remained low during the mid and late phases while
expression of OPN mRNA was maximum during
the early phase and declined sequentially during
the mid and late phase of oestrus cycle.
Description
TNV_BUFBUL_2022_41(2)251-261
Keywords
Veterinary Science