MOLECULAR DETECTION OF BEEF ADULTERATION IN MUTTON BY DUPLEX POLYMERASE CHAIN REACTION
Loading...
Date
2023
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
A duplex polymerase chain reaction (PCR) technique was evaluated for detection of beef
adulteration in mutton. A total of 22 fresh longissimus dorsi muscle samples each of mutton and
beef was collected in December, 2020 from 15 retail shops located in and around Chennai. Meat
sample was taken in different proportions (mutton :beef) as 100:0, 90:10, 80:20, 70:30, 60:40,
50:50 and 0:100. PCR amplified with species-specific primers for cytochrome b gene revealed
species-specific bands at 331 and 274 bp for mutton and beef, respectively. The primers used in
the study amplified the corresponding sequence of cytochrome b gene for the different proportions
of meat and beef samples. From the study, it can be concluded that duplex PCR is a reliable
technique to detect the adulteration of fresh and adulterated samples of mutton and beef.
Description
TNV_IJSR_2023_29(1)179-181
Keywords
Veterinary Science