RECEPTOR PHARMACOLOGY OF PHYTOL ON RAT INTESTINE
Loading...
Date
2022-02-15
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
COLLEGE OF VETERINARY AND ANIMAL SCIENCES POOKODE
Abstract
Receptors act as mediators between the drug molecule and the physiological
responses elicited. The activity of any compound depends on its interaction with the
intracellular and trans-membrane receptors. Receptor binding and functional assays
help in characterisation of novel receptors and receptor subtypes. Phytol is a
diterpenoid has gained importance in therapeutics recently because of its numerous
pharmacological activities. The studies on interaction of phytol with known classical
receptors are meagre in the literature. Therefore, the present study was undertaken to
assess the binding of phytol with the muscarinic, GABAnergic and histaminergic
receptors in rat ileum using a Digital Dales mono bath with isometric transducer. The
EC50 and IC50 of phytol in presence of agonist and antagonist of muscarinic
cholinergic, GABAergic and histaminergic receptors were derived. Pre-treatment
with submaximal doses of phytol attenuated the contractile response to acetylcholine
(ACh). The activity of phytol as a muscarinic blocker was comparable with that of
solifenacin while the relaxation potential for subsidence of pre-contracted intestine
with ACh was incomplete and less effective. The response of phytol in presence of
GABA and histamine receptor agonists and antagonists were not well appreciated. In
conclusion, phytol produced weak spasmolytic effect in the rat ileum mediated
through muscarinic receptors. Further, downstream pathway studies are required to
understand the mechanism by which phytol induced relaxation of the rat ileum.
Description
Submitted in partial fulfilment of the requirement for the degree of
MASTER OF VETERINARY SCIENCE in Veterinary Pharmacology and Toxicology