Comparative study on phosphate solubilization potential of bacteria in wheat under agroforestry vs open cultivation systems
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Date
2022-10
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G. B. Pant University of Agriculture and Technology, Pantnagar
Abstract
To fulfill the rising need for food, chemical fertilizers and pesticides have been used excessively, which has surely resulted in pollution and significant harm to soil fertility. The use of bio fertilizers, an environmentally benign agricultural input that is also more affordable than chemical fertilizers, significantly increases soil fertility over time. Phosphate solubilizing bacteria associated with plant increase the plant available phosphorus in soil thereby promoting the plant growth as well as yield. The current study examined the plant growth promoting potential of two PSB Pseudomonas strains on wheat through a field trial in open cultivation system and agroforestry system. Both the strains were previously isolated from the Dalbergia sissoo forest in the North-western Himalayan region of India and tested for their ability to serve as bio fertilizer using an integrative approach that included field trial with recommended dose of NPK. They were identified as Pseudomonas paralactis L3 (NCBI Gen Bank Accession number MG966341), Culture Accession number NAIMCC-B-03170 and Pseudomonas aeroginosa P2 (NCBI Gen Bank Accession number MG966347), Culture Accession number NAIMCC-B-03171). The field trial was conducted in two experimental setup i.e. open cultivar with Randomized Block Design (RBD) having three replicates per treatment and in all three treatments,P2, L3 individual and in consortium, CNS (L3+P2). At 60DPI and 120DPI in open field cultivation, CNS showed the best result in all agronomic parameters, GY, BY, HI and 1000 grain weight, photosynthetic pigments, microbial count and soil enzymatic activities and were significantly higher than untreated control at p<0.05. Whereas in agroforestry system P2 showed the best result. At 120DPI the highest GY (14.37qha-1), BY(16.27qha-1), HI (88.29) and1000grain yield (36g) was observed in P2 strain in agroforestry system. Whereas in open cultivation system GY (13.27qha-1), BY(15.06qha-1), HI (88.1) and 1000grain yield (33.33g) was shown by CNS. The highest NPKcontent in soil during open field cultivation was observed in CNS (37.30%,0.44% and 1.63%) and in agroforestry system the maximum response was observed in P2 (38.09%, 0.482% and 1.69%) as compared to uninoculated control. In open system the highest protein and carbohydrates content were found in CNS10.82% and 2.603% whereas in agroforestry system P2 showed the highest response 12.52% and 2.93%. To assess the impact of PSB on wheat seed germination the in vivo seed germination assay was performed in which P2 treated seeds showed significantly higher seed shoot length (SL) crawling as compared to untreated wheat seedling. Overall while comparing both the systems the best results were obtained in agroforestry system as compared to open field cultivation.