Effect of silicon dioxide nanoparticles on micropropagation and metabolomics of strawberry (Fragaria × ananassa duch.)
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Date
2022-03
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G. B. Pant University of Agriculture and Technology, Pantnagar
Abstract
Microbial contamination and oxidative stress induced damage are the two most limiting factors in micropropagation, which not only reduce the plantlet quality but also affect the survival rate during acclimatization. Nanoparticles (NPs) due to their unique properties exhibit broad spectrum antimicrobial activity against pathogens and also enhance the growth and development of plants by assisting in protection against abiotic stresses. The present investigation was carried out at G.B.P.U.A. & T., Pantnagar, during the year 2017-21, to study the various effects of SiO2 NPs on micropropagation of strawberry cv. Chandler. For explant sterilization, different concentrations of SiO2 NPs (50-1000 ppm) were either used through priming or directly inoculated in the MS medium. For growth studies, explants were multiplied in MS media supplemented with various concentrations of SiO2 NPs (50-200 ppm) along with PGRs. The results showed that lowest contaminate rate was observed in explants inoculated in MS medium containing SiO₂ NPs @ 200 ppm. Highest number and length of shootlets, highest number of roots, maximum shoot and root biomass and 100 per cent survival rate of plantlets during acclimatization was observed in plantlets obtained from 150 ppm SiO2 NPs incorporated growth medium. Improved RWC and enhanced production of chlorophyll, carotenoids, proline and antioxidant enzymes such as SOD, CAT, GPX, APX and GR activity was also observed in plantlets cultured in 150 ppm SiO2 NPs containing growth medium as compared to control. It was also evident from the metabolomics data, that plantlets obtained from 150 ppm SiO2 NPs media showed an increase in various sugars, amino acids and organic acids concentration as compared tocontrol during different developmental stages. Thus, it can be concluded that SiO2 NPs was highly effective in controlling contamination and enhanced the efficiency of micropropagation by reducing the cost of in vitro cultures and improving stress tolerance in plantlets as compared to conventional micropropagation methods.