SCREENING AND BIOCHEMICAL CHARACTERIZATION OF RICE BEAN (Vigna umbellata L.) ACCESSIONS FOR BEAN COMMON MOSAIC VIRUS
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Date
2022-04-11
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The survey conducted during 2019-20 in six districts of Southern Karnataka to assess the incidence of mosaic disease of cowpea, revealed the occurrence of disease in the range of 18.66 to 50 percent. Highest disease incidence was recorded in Bengaluru urban (50percent) and least disease incidence in Chikkabalapura district (18.66 percent). Infected cowpea was used as a source of Bean Common Mosaic Virus. The virus was infected to rice bean accessions through mechanical sap transmission. Presence of virus in the infected plant was confirmed by RT-PCR technique using primers specific for coat protein gene. Based on the extent of severity of symptoms, it was found that, 39 accessions were immune, EC-37 showed resistance reaction, EC-48, EC-34, IC-3 showed moderately resistance reaction, EC - 27, KBR 1 showed susceptible reaction. No accessions were highly susceptible. Rice bean accessions differing in the level of resistance were used further for biochemical studies. The total chlorophyll content, total sugars, phenolics were less in susceptible accessions. Total protein content was more in susceptible. Activity of antioxidant enzymes like peroxidase, catalase and Super oxide dismutase were high in infected plants and varied among the infected rice bean accessions.
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