UNDERSTANDING THE RELATEDNESS AMONG DIFFERENT ELEUSINE SPECIES USING SSR MARKERS DEVELOPED FROM Eleusine indica AND Eleusine coracana GENOME SEQUENCES
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Date
2022-01-19
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University of Agricultural Sciences, Bangalore
Abstract
In the present investigation, an attempt was made to understand the morphological and molecular relationship between 12 genotypes belonging to seven Eleusine species. Studies on 14 qualitative and 10 quantitative traits revealed greater diversity among wild species compared to cultivated Eleusine species. The principal component analysis revealed that the plant height, number of fingers on main ear, finger length, flag leaf length, flag leaf width, days to 50% flowering are the important traits that contribute most to the variation. The transfer potential of SSR markers showed 100% transferability rate in all the species under study except for E. multiflora and E. jaegeri. The lowest transferability was observed for E. multiflora i.e., 88% for E. indica SSRs, 92% for E. coracana SSRs and 86.7% for EST SSRs. Out of 85 SSR markers studied, 61 markers (72%) exhibited polymorphism. The number of alleles produced per primer varied from 1 to 5, major allele frequency ranged from 0.33 to 1, gene diversity ranged from 0 to 0.77, polymorphic information content ranged from 0 to 0.68. Dendrogram based on UGPMA clustering using morphological and molecular data grouped the genotypes into two major clusters. Cluster I was a solitary cluster with GE7143 (Eleusine multiflora) genotype and other was with remaining wild and cultivated genotypes. The results indicated that E. multiflora was found distinct from other species while, E. indica and E. coracana subsp africana were found
very close to E. coracana both morphologically and genetically which need to be exploited in interspecific hybridization.