MOLECULAR DIVERSITY ANALYSIS OF FERN SPECIES OF AGUMBE GHATS OF KARNATAKA USING ISSR MARKERS
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Date
2022-02-05
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Abstract
A survey of fern flora of Agumbe Ghats of Karnataka was undertaken to access the
diversity of ferns. The present study resulted in the collection of 12 species of pteridophytes belonging to 11 genera under 11 families. The herbaria were prepared for the present study and were deposited in Mahatma Gandhi Botanical Garden of GKVK, Bangalore. The slightly modified CTAB DNA extraction method with the addition of polyvinyl pyrrolidone (PVP), Phenol: chloroform: Isoamyl alcohol (PCI) yielded a good DNA concentration. 9 ISSR primers were selected to study the genetic diversity among 12 fern species. The annealing temperature for all ISSR primers was standardized by gradient PCR and the annealing temperature for all primers ranged from 42 to 56 °C. ISSR fragments generated 32 to 66 bands per primer. A total of 389 polymorphic bands produced 100 percent polymorphism per primer. The similarity coefficient between the species was within the range of 4 to 35 per cent. The dendrogram generated by ISSR markers revealed two major clusters, indicating that fern species have distributed based on frond shape, frond type, type of rhizome, habitat, stipe colour, texture. Based on molecular data the highest genetic similarity of 35 per cent was observed between Pteris confusa and Pteris biaurita. while Tectaria coadunata and Adiantum philippense showed the least genetic similarity index of 4 per cent.
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