Induction of androgenesis in head cabbage (Brassica oleracea var. capitata L.)
Loading...
Date
2022-11-02
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Palampur
Abstract
The present investigation entitled “Induction of androgenesis in head cabbage
(Brassica oleracea var. capitata L.)” was undertaken to assess the effect of different
duration of pretreatment (2 and 4 days at 35°C), culture media ( B5 and NLN),
sucrose concentrations (10 and 12 %) and hormonal combinations [2, 4-D (0.5 mg/l)
+ NAA (1.0 mg/l), 2, 4-D (0.5 mg/l) + NAA (1.5 mg/l), 2, 4-D (1.0 mg/l) + NAA (1.0
mg/l), 2, 4-D (1.0 mg/l) + NAA (1.5 mg/l)] on callus induction frequency and to
determine the effect of different growth regulators on callus regeneration. The
experiment was laid out in Completely Randomized Design (CRD) with three
replications for each treatment. The highest callus induction frequency was recorded
after 2 days of pre-treatment at 35°C on the B5 media supplemented with 10 per cent
sucrose and 2, 4-D (0.5 mg/l) + NAA (1.0 mg/l) hormonal combination. The highest
callus regeneration frequency was obtained when calli were transferred to B5 medium
containing 3 % sucrose and supplemented with 1.0 mg/l BAP + 2.0 mg/l Kinetin +
0.25 mg/l NAA hormonal combination. The rhizogenesis was induced in the shoots
derived from the callus which were sub-cultured on rooting medium (B5 + 3 %
sucrose), among the two different concentration of IBA tested 0.1mg/l gave higher
rooting percentage as compared to 0.2mg/l IBA.