Immobilization of xylanase from Bacillus pumilus over aluminum oxide beads
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Date
2021-09
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CCSHAU, Hisar
Abstract
In present investigation the xylanase from Bacillus pumilus SV-85S was covalently immobilized over aluminum oxide beads. The immobilization yield and efficiency were 48.05 and 83.13%, respectively. Some surface modifications were observed through SEM analysis on xylanase bound aluminum oxide beads. The bands at 3440 and 1635 cm-1 were observed through FTIR analysis over aluminum oxide beads, glutaraldehyde treated beads and immobilized xylanase. Optimum pH and temperature for free and immobilized enzyme were 7.0 and 55ᵒC, respectively. Increment of Km and Vmax values was observed in immobilized enzyme by around 28.96 and 34.61 % respectively. The pH, thermal and storage stability were improved after immobilization. The Immobilized xylanase retained 65 % of its initial activity even after 5 cycles of reusability. The increase in Half-life (t1/2) and D- value whereas decrease in kd value after immobilization, indicates that thermal stability of xylanase was enhanced. Thermodynamic parameters (ΔH, ΔG and ΔS) were enhanced after immobilization of xylanase. The decrease in activation energy in case of immobilized xylanase indicates that catalytic efficiency was enhanced. Hence, the present investigation concludes that immobilization of enzyme is important due to its considerable reusability and stability, and advantageous for industrial application.