Studies on clonal diversity in eucalyptus using biochemical and molecular approaches
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Date
2022-09
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G.B. Pant University of Agriculture and Technology, Pantnagar, District Udham Singh Nagar, Uttarakhand. PIN - 263145
Abstract
Eucalyptus is a fast growing evergreen tree with its rapid growth and capacity to satisfy rural needs for fuel and shelter, its plantations were widely encouraged. The current work details about the evaluation of the clonal diversity through biochemical and molecular parameters among five Eucalyptus clones acquired from Agro Forestry Research Centre, GBPUAT, Pantnagar. Total phenol content was observed to be lowest (109.6±0.05 mg GAE/g) in sample PES-21-4 and highest (132±0.09 mg GAE/g) in sample K 25. Total antioxidant capacity was observed to be lowest (15.22±0.006 mg/g) in sample PES-21-3 and highest (17.89±0.02 mg/g) in sample PES-21-2. Total flavonoid content was observed to be lowest (19.48±0.2 mg CE/g) in sample PES-21-3 and highest (24.81±0.3 mg CE/g) in sample PES-21-1. Total tannin content was observed to be lowest (9.12±0.05 TAE/g) in sample PES- 21-1 and highest (10.17±0.13 TAE/g) in sample PES-21-4. IC50 value of DPPH activity was observed to be lowest (23.49±0.009 μg/ml) in sample PES-21-3 and highest (26.01±0.005 μg/ml) in sample K25. IC50 value of metal ion chelating activity was observed to be lowest (17.76±0.11 μg/ml) in sample PES-21-3 and highest (19.86±0.03 μg/ml) in sample K25. Amylase inhibition was found to be highest in hexane extract (46.04±0.11%) than the methanolic extract (34.59±0.03 %). SDS-PAGE analysis revealed 15 bands out of which the most prominent band obtained was band 14 of size 92.7kDa with Rf value of 0.277. Five Eucalyptus clones were characterized at molecular level using eight RAPD primers. The minimum similarity coefficient was between clone PES-21-1 and clone PES-21-4 (0.63), while maximum similarity coefficient was between clone PES-21-3 and clone PES-21-4 (0.79). Dendogram revealed that Clone PES-21-3 and clone PES-21-4 are showing high similarity while Clone K 25 was quite diverse from rest of the clones. The quantitative determination of the phytochemical composition and their activities in Eucalyptus helps to understand its therapeutic properties and utilizing molecular markers to determine the clonal diversity leads to a better knowledge about plant evolutionary history and provide valuable information about agrobiodiversity dynamics.