VARIETAL RESPONSE IN ROSE FOR MICROPROPAGATION
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Date
2017-06
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University of Horticultural Sciences, Bagalkot. (College of Horticulture, Bengaluru).
Abstract
The present study on “Varietal response in rose for micropropagation” was conducted to
assess the response of normal and irradiated explants of rose genotypes Arka Swadesh and
IIHRR7-1 to in vitro culture. The nodal segments were cultured on MS medium supplemented
with different hormonal combinations for shoot regeneration. The hormonal combination BAP
3ppm + GA3 0.5ppm was considered as best for shoot proliferation of both the genotypes with
much more pronounced effect in Arka Swadesh. In these hormonal combinations, early shoot
initiation (8 days) was observed. The longer shoots (2.03cm), more number of branches per
shoot (4.80) and leaves per shoot (20.60) and best response for multiple shoot production was
also observed in same media combination. The hormonal combinations NAA (0.1 and 0.2ppm)
had taken more number of days for shoot initiation. Incorporation of NAA in media resulted in
reduced length of shoots, less number of branches and leaves per shoot. Incorporation of NAA
either 0.1ppm or 0.2ppm in combination with GA3 (0.5ppm) resulted in inhibition of shoot
formation in both the genotypes. Half strength MS medium supplemented with IBA at 0.5ppm
resulted in good rooting in both genotypes. In case of gamma irradiated explants, the irradiation
dosage of 0.05 K Gy was determined as LD50 dose based on the explants survival percentage.
The highest rate of explants survival (100%), longer shoots (1.93cm), more number of branches
per shoot (4.75) leaves per shoot (19.20) was observed in unexposed explants. At 0.05 K Gy
dosage, shorter shoots length (1.18cm) and minimum number of branches (3.50) per shoot and
leaves per shoot (12.25) were observed. The research established the significance of hormonal
combinations for organogenesis in rose. Standardized micropropagtion protocol in combination
with standardization of LD50 dosage can further be utilized efficiently for rose breeding program
as well as mass propagation.