VARIETAL RESPONSE IN ROSE FOR MICROPROPAGATION
| dc.contributor.advisor | TEJASWINI | |
| dc.contributor.advisor | G. K., SEETHARAMU | |
| dc.contributor.author | M. R., VINUTHA | |
| dc.contributor.other | J. B., MYTHILI | |
| dc.contributor.other | P. M., MUNIKRISHNAPPA | |
| dc.date.accessioned | 2022-08-24T10:49:24Z | |
| dc.date.available | 2022-08-24T10:49:24Z | |
| dc.date.issued | 2017-06 | |
| dc.description.abstract | The present study on “Varietal response in rose for micropropagation” was conducted to assess the response of normal and irradiated explants of rose genotypes Arka Swadesh and IIHRR7-1 to in vitro culture. The nodal segments were cultured on MS medium supplemented with different hormonal combinations for shoot regeneration. The hormonal combination BAP 3ppm + GA3 0.5ppm was considered as best for shoot proliferation of both the genotypes with much more pronounced effect in Arka Swadesh. In these hormonal combinations, early shoot initiation (8 days) was observed. The longer shoots (2.03cm), more number of branches per shoot (4.80) and leaves per shoot (20.60) and best response for multiple shoot production was also observed in same media combination. The hormonal combinations NAA (0.1 and 0.2ppm) had taken more number of days for shoot initiation. Incorporation of NAA in media resulted in reduced length of shoots, less number of branches and leaves per shoot. Incorporation of NAA either 0.1ppm or 0.2ppm in combination with GA3 (0.5ppm) resulted in inhibition of shoot formation in both the genotypes. Half strength MS medium supplemented with IBA at 0.5ppm resulted in good rooting in both genotypes. In case of gamma irradiated explants, the irradiation dosage of 0.05 K Gy was determined as LD50 dose based on the explants survival percentage. The highest rate of explants survival (100%), longer shoots (1.93cm), more number of branches per shoot (4.75) leaves per shoot (19.20) was observed in unexposed explants. At 0.05 K Gy dosage, shorter shoots length (1.18cm) and minimum number of branches (3.50) per shoot and leaves per shoot (12.25) were observed. The research established the significance of hormonal combinations for organogenesis in rose. Standardized micropropagtion protocol in combination with standardization of LD50 dosage can further be utilized efficiently for rose breeding program as well as mass propagation. | en_US |
| dc.identifier.other | UHS15PGM654 | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810186645 | |
| dc.keywords | Horticulture, Floriculture, Landscape Architecture, Micropropagation, Genotypes, Arka Swadesh, Nodal Segments, Proliferation, Regeneration, Organogenesis, Irradiation, Protocol, UHS15PGM654, T320. | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 83p. (Due to pdf. there is a difference in pages in soft copy and hard copy) | en_US |
| dc.publisher | University of Horticultural Sciences, Bagalkot. (College of Horticulture, Bengaluru). | en_US |
| dc.sub | Floriculture and Landscaping | en_US |
| dc.theme | VARIETAL RESPONSE IN ROSE FOR MICROPROPAGATION | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | VARIETAL RESPONSE IN ROSE FOR MICROPROPAGATION | en_US |
| dc.type | Thesis | en_US |