Comparative studies of susceptible and tolerance varieties of pigeon pea (Cajanus cajan) using protein profiling and RAPD markers with response to Pod borer (Helicoverpa armigera) resistance
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Date
2016-07-11
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ANDUAT, Kumarganj, Ayodhya
Abstract
Pigeonpea seeds are important source of proteins, energy and other nutrients
in the diets of large population groups around the world, forming and excellent
source of lysine, methionine and tryptophan and other water soluble vitamins
(riboflavin, niacin and folacin) and of the minerals phosphorus, iron and magnesium
(Ramcharan and Walker, 1985).
The present study involved evaluation of ten genotypes of pigeonpea
including five susceptible and five resistant varieties viz., NDA-1, NDA-2, Bahar,
NDA-14-6, NDA-13-6, NDA-3, UPAS-120, MAL-6, MAL-13, Cajanus
scarabaeoides (wild). The work was done with 10 %, 12 %, 15 % protein profiling,
12 % native gel analysis, DNA isolation and RAPD analysis. The result obtained on
above characters was analyzed for protein profiling and RAPD analysis. Gel
electrophoresis of protein of pigeonpea varieties show novel band obtain at the upper
portion of the 10 % acrylamide gel , these band only seen 0.455 (RM value) is pod
borer tolerant and regarded as pod borer resistant responsible protein.
RAPD primer was initially tested for the PCR amplification of the genomic
DNA for 10varieties, but they did not amplify genomic DNA. Genomic DNA was
amplified with RAPD specific primer i.e OPA-15, OPU-11 and OPA-16, but no
result was found. DNA and RAPD analysis was the best tools for identification of
resistant and susceptible gene for podborer (Helicoverpa armigera).
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