Horticultural and molecular evaluation of tomato lines for multiple disease resistance
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Date
2021
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Punjab Agricultural University, Ludhiana
Abstract
Tomato (Solanum lycopersicum L.) can be grown throughout the year but its production is
hampered due to attack of several diseases. In tropics and subtropics, tomato leaf curl disease
(ToLCD), late blight (LB) and root knot nematode (RKN) are the major diseases, which can
cause 90-100 percent losses of yield and quality. Hence, six genes (Ty-1, Ty-2, Ty-3, Ph-2,
Ph-3 and Mi-1.2) were pyramided from different parents to accomplish resistance against
ToLCD, LB and RKN. In the present investigation, 132 lines of BC2F6 population were
screened for resistance to multiple diseases along with horticultural traits. The popular
released cultivar ‘Punjab Chhuhara’ was used as a common susceptible control for all the
three diseases and resistant controls include Punjab Varkha Bahar-4 for ToLCD, LBR-10 for
LB and NR-14 for RKN. Natural, artificial and marker aided screening were carried out for
identification of disease resistant lines. In 2019-20, horticultural evaluation of advance
breeding lines along with screening for multiple disease resistance under natural and artificial
conditions was done. During 2020-21, marker assisted screening against diseases was
employed. Out of 132 lines screened, 5 lines viz. PDRT-104, PDRT-118, PDRT-122, PDRT124
and PDRT-125 were found to be resistant against all the three diseases viz. ToLCD, LB
and RKN along with better horticultural traits. The lines namely PDRT-145 and PDRT-154
possessing resistance against ToLCD and RKN and the line PDRT-1 possessing resistance
against LB and RKN. Among these lines, PDRT-104 and PDRT-122 were found to be
possessing all the six genes (Ty-1, Ty-2, Ty-3, Ph-2, Ph-3 and Mi-1.2) with average yield of
3.07 and 3.44 kg/plant, respectively. Whereas, the lines PDRT-124 and PDRT-125 were
found to possesses five genes (Ty-1, Ty-2, Ty-3, Ph-2 and Mi-1.2) with average yield of 3.04
and 2.78 kg/plant, respectively. The multiple disease resistant lines found in the present study
could be evaluated at multi-site for their commercial exploitation and can also be considered
as potential candidates in resistance breeding programmes of tomato crop.
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Citation
Rane Omkar Harikrishna (2021). Horticultural and molecular evaluation of tomato lines for multiple disease resistance(Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.