Horticultural and molecular evaluation of tomato lines for multiple disease resistance

dc.contributor.advisorJindal, Salesh Kumar
dc.contributor.authorRane Omkar Harikrishna
dc.date.accessioned2022-02-04T04:48:56Z
dc.date.available2022-02-04T04:48:56Z
dc.date.issued2021
dc.description.abstractTomato (Solanum lycopersicum L.) can be grown throughout the year but its production is hampered due to attack of several diseases. In tropics and subtropics, tomato leaf curl disease (ToLCD), late blight (LB) and root knot nematode (RKN) are the major diseases, which can cause 90-100 percent losses of yield and quality. Hence, six genes (Ty-1, Ty-2, Ty-3, Ph-2, Ph-3 and Mi-1.2) were pyramided from different parents to accomplish resistance against ToLCD, LB and RKN. In the present investigation, 132 lines of BC2F6 population were screened for resistance to multiple diseases along with horticultural traits. The popular released cultivar ‘Punjab Chhuhara’ was used as a common susceptible control for all the three diseases and resistant controls include Punjab Varkha Bahar-4 for ToLCD, LBR-10 for LB and NR-14 for RKN. Natural, artificial and marker aided screening were carried out for identification of disease resistant lines. In 2019-20, horticultural evaluation of advance breeding lines along with screening for multiple disease resistance under natural and artificial conditions was done. During 2020-21, marker assisted screening against diseases was employed. Out of 132 lines screened, 5 lines viz. PDRT-104, PDRT-118, PDRT-122, PDRT124 and PDRT-125 were found to be resistant against all the three diseases viz. ToLCD, LB and RKN along with better horticultural traits. The lines namely PDRT-145 and PDRT-154 possessing resistance against ToLCD and RKN and the line PDRT-1 possessing resistance against LB and RKN. Among these lines, PDRT-104 and PDRT-122 were found to be possessing all the six genes (Ty-1, Ty-2, Ty-3, Ph-2, Ph-3 and Mi-1.2) with average yield of 3.07 and 3.44 kg/plant, respectively. Whereas, the lines PDRT-124 and PDRT-125 were found to possesses five genes (Ty-1, Ty-2, Ty-3, Ph-2 and Mi-1.2) with average yield of 3.04 and 2.78 kg/plant, respectively. The multiple disease resistant lines found in the present study could be evaluated at multi-site for their commercial exploitation and can also be considered as potential candidates in resistance breeding programmes of tomato crop.en_US
dc.identifier.citationRane Omkar Harikrishna (2021). Horticultural and molecular evaluation of tomato lines for multiple disease resistance(Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810181685
dc.keywordsTomato, Advance Breeding Lines, Tomato Leaf Curl Disease, Late Blight, Root Knot Nematode, Multiple Disease Resistanceen_US
dc.language.isoEnglishen_US
dc.pages92en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.rating.count5en
dc.research.problemHorticultural and molecular evaluation of tomato lines for multiple disease resistanceen_US
dc.subVegetable Scienceen_US
dc.themeHorticultural and molecular evaluation of tomato lines for multiple disease resistanceen_US
dc.these.typeM.Scen_US
dc.titleHorticultural and molecular evaluation of tomato lines for multiple disease resistanceen_US
dc.typeThesisen_US
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