Molecular characterization of HSP108 gene in local chicken of Poonch
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Date
2022-01-17
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Sher-e-Kashmir University of Agricultural Sciences & Technology, Jammu (J&K)
Abstract
The present study on molecular characterization of heat shock protein 108 (HSP108) gene in local chicken of Poonch was undertaken with the objective of amplification and sequencing of HSP108 gene and to study the genetic similarity as well as distance between different species. Total RNA was extracted from 2ml freshly collected chicken blood using all blood RNA purification kit. cDNA was synthesized by reverse transcription from RNA using RevertAid First Strand cDNA Synthesis kit. HSP108 gene was amplified from cDNA using specific primers designed by Primer3 software. The amplified product was purified by GenElute gel extraction kit and thereafter cloned and sequenced. Coding sequence (CDS) of HSP108 gene was 2388 bp that encoding 795 amino acids. On its comparison, chicken CDS demonstrated 97.24, 96.94, 96.61, 93.94, 83.23, 83.13, 82.29, 82.85, 82.76, 82.49 and 82.44 percent homology with turkey, quail, guinea fowl, duck, horse, camel, pigs, cattle, buffalo, sheep and goat, respectively. Indicates close evolutionary relationship and high sequence homology among the species. Deduced amino acid sequence of 795 residues of HSP108 gene was 98.82, 97.51, 52.13, 51.49, 51.15, 48.22, 47.86, 47.86, 47.86, 47.86, 47.66 and 47.62 percent similar to chicken, duck, pig, buffalo, camel, goat, quail, turkey, sheep, horse, cattle and guinea fowl, respectively. Phylogenetic analysis revealed that chicken CDS sequence was close to turkey, followed by quail, guinea fowl and duck but it was most divergent from equines. It may also be inferred that this gene might have been evolved by purifying selection. From the present study, it can be concluded that chicken HSP108 is highly conserved among different species.