Molecular characterization of HSP108 gene in local chicken of Poonch

Simple item page
dc.contributor.advisorMahajan, Vikas
dc.contributor.authorSingh, Vishav Jeet
dc.date.accessioned2022-01-19T04:39:44Z
dc.date.available2022-01-19T04:39:44Z
dc.date.issued2022-01-17
dc.description.abstractThe present study on molecular characterization of heat shock protein 108 (HSP108) gene in local chicken of Poonch was undertaken with the objective of amplification and sequencing of HSP108 gene and to study the genetic similarity as well as distance between different species. Total RNA was extracted from 2ml freshly collected chicken blood using all blood RNA purification kit. cDNA was synthesized by reverse transcription from RNA using RevertAid First Strand cDNA Synthesis kit. HSP108 gene was amplified from cDNA using specific primers designed by Primer3 software. The amplified product was purified by GenElute gel extraction kit and thereafter cloned and sequenced. Coding sequence (CDS) of HSP108 gene was 2388 bp that encoding 795 amino acids. On its comparison, chicken CDS demonstrated 97.24, 96.94, 96.61, 93.94, 83.23, 83.13, 82.29, 82.85, 82.76, 82.49 and 82.44 percent homology with turkey, quail, guinea fowl, duck, horse, camel, pigs, cattle, buffalo, sheep and goat, respectively. Indicates close evolutionary relationship and high sequence homology among the species. Deduced amino acid sequence of 795 residues of HSP108 gene was 98.82, 97.51, 52.13, 51.49, 51.15, 48.22, 47.86, 47.86, 47.86, 47.86, 47.66 and 47.62 percent similar to chicken, duck, pig, buffalo, camel, goat, quail, turkey, sheep, horse, cattle and guinea fowl, respectively. Phylogenetic analysis revealed that chicken CDS sequence was close to turkey, followed by quail, guinea fowl and duck but it was most divergent from equines. It may also be inferred that this gene might have been evolved by purifying selection. From the present study, it can be concluded that chicken HSP108 is highly conserved among different species.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810180974
dc.keywordsHSP108 GENE, MOLECULAR CHARACTERIZATION, SEQUENCING, HOMOLOGY, PURIFYING SELECTION, POONCHI CHICKENen_US
dc.language.isoEnglishen_US
dc.pages74pen_US
dc.publisherSher-e-Kashmir University of Agricultural Sciences & Technology, Jammu (J&K)en_US
dc.research.problemMolecular characterization of HSP108 gene in local chicken of Poonchen_US
dc.subAnimal Genetics and Breedingen_US
dc.themeMolecular characterization of HSP108 gene in local chicken of Poonchen_US
dc.these.typeM.V.Sc.en_US
dc.titleMolecular characterization of HSP108 gene in local chicken of Poonchen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Thesis Vishav Jeet Singh.pdf
Size:
9.69 MB
Format:
Adobe Portable Document Format
Description:
Molecular characterization of HSP108 gene in local chicken of Poonch
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Theses