EVALUATION OF GREEN GRAM (Vigna radiata(L.) R. Wilczek) LINES FOR YMV RESISTANCE USING MOLECULAR MARKERS MOHD ABDUS SUBHAN
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Date
2020
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PROFESSOR JAYASHANKAR TELANGANA STATE AGRICULTURAL UNIVERSITY
Abstract
Green gram (Vigna radiata (L.) Wilczek), is one of the important pulse crops mainly grown in developing countries. However, the yield level of the crop is very low due to many biotic and abiotic factors. Among biotic factors, yellow mosaic virus (YMV), which is transmitted by white fly (Bemisiatabaci) causes significant yield losses ranging from 10-100% and it leads to severe yield reduction. The biggest challenge in YMD management is the effective utilization of an array of information gained so far, in an integrated manner for the development of genotypes having durable resistance against yellow mosaic virus (YMV) infection. The advancements in the field of biotechnology and molecular biology such as marker assisted selection and genetic transformation can be utilized in developing Yellow mosaic virus (YMV) resistant green gram.
The present investigation was carried out for screening of green gram RILs against Yellow mosaic virus (YMV) and evaluation based on morphological characters and molecular markers. An attempt was made to evaluate microsatellite markers linked to the YMV resistance in a F6 generation of green gram. The genotypes, MGG 295, susceptible to Yellow Mosaic Virus (YMV) and WGG 42 (Yadadri) resistant to YMV were chosen as parents for development of F6 population. The studies were carried out at Institute of Biotechnology (IBT), Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad and ARS Madhira, Khammam during 2019-2020 with 128 F6 RILs to elicit the information on nature and extent of the genetic variability, heritability, genetic advance and molecular evaluation for YMV.For molecular marker evaluation studyof 128 F6 RILs, SSR primers were employed. Observations were recorded on 13 characters viz., days to initial flowering, days to 50% flowering, days to maturity, number of branches per plant, number of pods per cluster, number of cluster per plant, number of pods per plant, number of seeds per pod, plant height, pod length, seed yield per plant,100seed weight and percent of disease incidence under natural incidence of whitefly at hot spot.
Results obtained conveyed that genetic variability was present for all the characters studied indicating that the RILs represented wide variability. The genotypic coefficients of variation for all the characters studied were lesser than the phenotypic coefficients of variation indicating the modifying effects of the environment in association with the characters at genotypic level. High PCV and GCV estimates was noticed for number of pods per plant, seed yield per plant, number of cluster per plant and number of pods per cluster. High heritability along with high genetic advance as percent of mean was observed for number of pods per plant, seed yield per plant, number of cluster per plant, number of pods per cluster, number of branches per plant, number of seeds per pod and plant height indicating the role of additive genes in governing the inheritance of these traits and could be improved through selection. The traits seed yield per plant, number of pods per plant, number of clusters per plant and number of pods per cluster had recorded high PCV, GCV, high heritability along with high genetic advance as percent of mean indicated these traits were less influenced by environment and possess high genetic variability.Hence these RILs would be suitable for green gram breeding programme to develop improved varieties.
The parental DNA was extracted and screened with 185 microsatellite markers to detect the polymorphic markers. Out of these markers, 102 were amplified and 83 markers were not amplified. Of these 102 amplified markers, 15 primers showed polymorphism (14.7%) between the parents, and the rest of the markers were found to be monomorphic. All the 128 F6 lines were genotyped with the 8 polymorphic markers. CEDG228 was found to be significantly associated with the YMV resistance in green gram. Identification of molecular marker associated with resistance gene in the present study, will increase the efficiency and accuracy in YMV-resistance breeding program and this marker can be used in future for the development of high yielding YMV resistant cultivars in green gram.
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D10,629