EFFECT OF VITAMIN B12 (CYANOCOBALAMIN) PRIMING ON ALLEVIATING SALINITY STRESS IN CHICKPEA (Cicer arietinum L.)
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Date
2018-07-01
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University of Agricultural Sciences, Bangalore
Abstract
Chickpea (Cicer arietinum L.) is sensitive to salinity. The present investigation
was carried out to evaluate the effects of saline water and vitamin B12 (Cyanocobalamin) priming on growth parameters and biochemical attributes in chickpea genotypes HC-5 (salt sensitive) and JG-11 (salt tolerant). The seeds were primed in various concentrations of vitamin B12 (10 and 20 μM). Physiological characters (Plant height, number of leaves, dry weight, and relative water content) and biochemical (antioxidants, malondialdehyde, proline, protein) parameters were analyzed. Results revealed an increase in growth parameters such as plant height, number of leaves, root and shoot dry weight, leaf area and nodule number. Salinity stress caused a decline in yield and yield attributes in HC-5 genotype. At the biochemical level, higher accumulation of osmotic solute proline and lipid peroxidation were observed. An increase in the activity of Superoxide dismutase, Peroxidase and Catalase and increased leghaemoglobin content were also observed in tolerant genotype (JG-11) as compared to sensitive genotype. Seed priming with vitamin B12 induced these stimulatory effects on all the measured parameters. The relative expression of CMO and BADH gene was higher in JG-11 indicating increase in synthesis of glycine betaine in tolerant variety. SDS-PAGE revealed more deletion than addition of polypeptide bands and increase/decrease in the intensities of certain bands was noticed in the seeds of JG-11 and HC-5. Results evinced that Vitamin B12 priming of seeds can be effectively used as a pre-sowing treatment for mitigating adverse effects of salinity in chickpea genotypes.