IDENTIFICATION OF EHRLICHIA CANIS BY PCR WITH PHYLOGENETIC ANALYSIS IN DOGS FROM SOUTH INDIA
Loading...
Date
2020-03
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Canine monocytic ehrlichiosis (CME) or canine hemorrhagic fever is a potentially fatal tick-borne disease in tropical countries associated
with its deleterious effect on the health of dogs. Clinically affected (n=130) and apparently healthy dogs (n=20) for the purpose of deworming or
vaccination brought to Teaching Veterinary Clinical Complex (TVCC), Veterinary College and Research Institute (VC &RI), Namakkal were
screened for E. canis by microscopic examination and polymerase chain reaction assay. The PCR assay revealed a positivity of 8.0 percent samples
for E. canis targeting Vir-B9 gene and generating an amplicon of 380 bp. However, conventional microscopic blood smear examination failed to
detect E. canis owing to the low parasitaemia. The sequence of E. canis had a 100.0 per cent homology with the E. canis of dogs in India and USA. The
tick vector from infected dogs was identified as Rhipicephalus sanguineus. Dogs below1 year age group, females and pure breeds were found to be
more affected.
Description
TNV_HV_2020_59(S1)79
Keywords
Veterinary Science