Evaluation of the Probiotic Mixture and Bacitracin Methylene Disalicylate Supplementation on Intestinal Health of Clostridium perfringens Induced Necrotic Enteritis in Broiler Chickens
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Date
2021-02
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Abstract
An experiment was carried out to investigate the efficacy of probiotic GalliproTect® and
bacitracin methylene disalicylate (BMD) on improving intestinal health
against C.perfringens induced necrotic enteritis (NE) in broilers. Five hundred 500 day-old
broiler chicks (Cobb 400) were divided into five treatment groups, each with five
replicates of 20 chicks each by following a completely randomized design. The treatments
were an uninfected control (T1), an infected control (T2), an infected group supplemented
with probiotic GalliproTect®at 500 g/tone of feed (T3), containing 2×1010 cfu/g, an
infected group supplemented with BMD at 500 g/tone of feed (T4) and an infected group
supplemented with probiotic + BMD each at 500 g/tone of feed (T5). Necrotic enteritis
was induced in the broilers by oral inoculation of 30,000 sporulated Eimeria necatrix
oocysts on day 14 followed by Clostridium perfringens inoculation 1.0 mL (108cfu/mL) on
day 19 to 21 in group T3, T4 and T5.On day 28 (7thday post infection) scoring of gross
lesions were performed. Illeal mucosal samples were collected for mRNA quantification of
TLR and cytokine gene by real time PCR. Histological scores revealed that intestinal
necrotic lesions and inflammatory changes were reduced. Toll like receptors (TLRs) and
cytokine gene expression revealed that probiotic group found to increase interleukins
levels and decrease TLR 2 levels in necrotic enteritis infected chickens. Thus, the results
suggested that the probiotic supplementation could able to regulate the intestinal mucosal
immune response and there by ameliorate inflammation by altering the cytokine and TLR
gene expression.
Description
TNV_IJCMAS_2021_10(2)265-270
Keywords
Veterinary Science