ABILITY OF INDIAN STREET RABIES VIRUS ISOLATES TO INDUCE APOPTOSIS BY IN- VITRO
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Date
2015-01
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Abstract
An attempt had been made in this study to know the ability of Indian Street rabies virus isolates to
induce apoptosis in murine neuroblastoma cells. Thirty Indian street virus isolates were tested by FAT
among which 20 samples were positive which were used to infect MNA cells. Four street rabies virus
isolates and CVS were used in this study to show their ability to induce apoptosis. CVS used as
control in the amplification as well as in the demonstration of apoptosis. The expression of Caspase-
1, Bad and glycoprotein genes in the MNA cells in comparision with CVS was measured by using
densitometer. Street virus isolates expressed higher levels of Caspase-1 and Bad in comparison with
CVS. Further TUNEL staining method showed higher apoptotic index when compared to CVS by
Flow Cytometer reading. This study is revealed that Indian rabies street virus isolates can induce
apoptosis in murine neuroblastoma cells.
Description
TNV_33_ISVM_22-24Jan_(2015)9
Keywords
Veterinary Science