Characterization of mungbean mutant lines for YMD resistance and key agronomic traits
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Date
2020
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Punjab Agricultural University, Ludhiana
Abstract
The present study was undertaken to evaluate mungbean mutant lines for key agronomic traits
and yellow mosaic disease (YMD) resistance across different seasons i.e. summer and kharif
and for the identification of putative genes for YMD resistance in these lines. The
experimental material consisted of 21 mutant lines developed from irradiation of seeds of
mungbean varieties SML 668, SML 832 and TMB 37. A wide range of variability was
observed for plant height, number of clusters per plant, number of pods per plant, number of
seeds per pod, 100 seed weight, biological yield, economical yield and harvest index. MML
2637 and MML 2664 were identified as best mutant lines for summer season, while MML
2593 and MML 2664 were identified as best mutant linesfor kharif seasons respectively.
MML 2629 and MML 2664 showed potential as dual season lines. All the mutants were
highly resistant to YMD. Further, QTLs/genes/markers for Begomovirus resistance were used
to identify homologous accessions on mungbean genome. On prediction of protein domains
of these accessions, most common motifs were found as NB-ARC, TIR, RdRP and SGS3. A
set of 48 primers specific for RdRP and SGS3 gene families were designed and validated to
amplify complete coding sequences in resistant mutant and susceptible parent. The
presence/absence and sequence polymorphism indicated possible role of these two genes in
confering YMD resistance in mungbean.
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Mandahal, Kamalpreet Singh (2020). Characterization of mungbean mutant lines for YMD resistance and key agronomic traits (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.