Characterization of mungbean mutant lines for YMD resistance and key agronomic traits

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dc.contributor.advisorBains, T. S.
dc.contributor.authorMandahal, Kamalpreet Singh
dc.date.accessioned2021-02-09T06:43:10Z
dc.date.available2021-02-09T06:43:10Z
dc.date.issued2020
dc.description.abstractThe present study was undertaken to evaluate mungbean mutant lines for key agronomic traits and yellow mosaic disease (YMD) resistance across different seasons i.e. summer and kharif and for the identification of putative genes for YMD resistance in these lines. The experimental material consisted of 21 mutant lines developed from irradiation of seeds of mungbean varieties SML 668, SML 832 and TMB 37. A wide range of variability was observed for plant height, number of clusters per plant, number of pods per plant, number of seeds per pod, 100 seed weight, biological yield, economical yield and harvest index. MML 2637 and MML 2664 were identified as best mutant lines for summer season, while MML 2593 and MML 2664 were identified as best mutant linesfor kharif seasons respectively. MML 2629 and MML 2664 showed potential as dual season lines. All the mutants were highly resistant to YMD. Further, QTLs/genes/markers for Begomovirus resistance were used to identify homologous accessions on mungbean genome. On prediction of protein domains of these accessions, most common motifs were found as NB-ARC, TIR, RdRP and SGS3. A set of 48 primers specific for RdRP and SGS3 gene families were designed and validated to amplify complete coding sequences in resistant mutant and susceptible parent. The presence/absence and sequence polymorphism indicated possible role of these two genes in confering YMD resistance in mungbean.en_US
dc.identifier.citationMandahal, Kamalpreet Singh (2020). Characterization of mungbean mutant lines for YMD resistance and key agronomic traits (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810161030
dc.keywordsMungbean, mutant lines, variability, YMD, RdRP, SGS3en_US
dc.language.isoEnglishen_US
dc.pages87en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemCharacterization of mungbean mutant lines for YMD resistance and key agronomic traitsen_US
dc.subGenetics and Plant Breedingen_US
dc.themeCharacterization of mungbean mutant lines for YMD resistance and key agronomic traitsen_US
dc.these.typeM.Scen_US
dc.titleCharacterization of mungbean mutant lines for YMD resistance and key agronomic traitsen_US
dc.typeThesisen_US
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