EVALUATION OF THERAPEUTIC POTENTIAL OF PROBIOTIC LACTIC ACID BACTERIA ISOLATED FROM ETHNIC FERMENTED FOODS OF HIMACHAL PRADESH
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Date
2020-11
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NAUNI,UHF
Abstract
ABSTRACT
The present investigation was carried out to evaluate the potential probiotic lactic acid bacteria from
traditional food matrices of Himachal Pradesh, their screening, identification, assessment of essential probiotic
attributes and then finally their application as therapeutic agent. In total, 3 in-house lactic acid bacteria strains
isolated from 3 different food matrices viz. Chakti, Makhan and Dahi were selected for the evaluation of
bacterial isolates for their therapeutic potential as probiotics. Safety assessment of the isolates was done by
evaluating haemolytic, DNase, gelatinase activities and antibiotic susceptibility. All the three isolates exhibited
non-heamolytic, non-DNase and non-gelatinase activities and were highly sensitive to all the antibiotics except
that of Vancomycin, thereby proving overall safe status. These screened isolates were further assessed for their
probiotic potential viz. acidity tolerance, bile tolerance, auto-aggregation, co-aggregation, hydrophobicity,
antagonistic activity against test indicators, antioxidant activity, lipid peroxidation inhibition in liposome
system, adhesion to gastric mucin, inhibition, displacement as well as competitive exclusion of pathogens,
simulated gastro-intestinal transit, antagonistic spectrum, bacteriocin production, cholesterol lowering property,
and cumulative probiotic potential. All the three screened isolates were highly acidity tolerant with 87.2295.97%
survival rate at pH 3 for 3 h. These three isolates were also able to resist high bile salt concentration i.e.
2.0% with 80.74-89.00% survival rate for 8 h. All the three isolates exhibited good auto-aggregation capacity
i.e. > 40% after 5 h and have shown strong hydrophobicity towards xylene i.e. > 40%. Antioxidant activity of
the three isolates was in a range from 59.19-87.17%. Lipid peroxidation inhibition activity in the liposome
system also ranged from 51.87-90.41%. All the isolates have the ability to adhere gastric mucin with 20.5661.68%
adhesion and all of them have exhibited adhesion potential to gastric mucin. Resistance GI transit was
lied in a range from 48.51-66.01% at pH 3 and 68.04-73.81 at pH 8, both after 4 h. Further screening was
doneby bit/disc method and the three strains exhibited wide inhibitory spectrum against the test indicators.
These screened probiotic isolates showed strong inhibitory spectrum against both gram positive as well as gram
negative pathogenic bacteria through secretion of bacteriocin (24.25-26 mm zone size). Cholesterol assimilation
by the three LAB strains was evaluated and it ranged from 47.17-78.40%. All the screened isolates were found
to be qualified with quite an impressive cumulative score as high as 87.5%. Hence, this study affirms the use of
Lactobacillus fermentum Cha4, Lactobacillus delbreukii F18 and Lactobacillus rhamnosus KC6 in the
development of new therapeutic compounds to impart betterment to public health as all the strains under present
study have been proved safe as well as highly effective probiotic candidates.