STUDIES ON CULTIVATION, ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES OF LION’S MANE MUSHROOM (Hericium erinaceus)
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Date
2020-10
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NAUNI,UHF
Abstract
ABSTRACT
The present investigation “Studies on cultivation, antioxidant and antimicrobial properties of
Lion’s mane mushroom (Hericium erinaceus)” was under taken to evaluate various cultural requirements,
spawn preparation, cultivation, antioxidant and antibacterial properties of the test fungus. H. erinaceus is a
Chinese edible and medicinal mushroom and out of five solid media tested, potato dextrose agar was the best
solid media with fastest growth. Out of five carbon sources tested glucose was found to be best carbon source
with radial growth of 40.00 mm and alanine gave the best growth (39.87 mm) among all five nitrogen sources
tested. Optimum temperature for H. erinaceus was found to be 250C and it gave best growth at pH 6 among all
the pH values tested. H. erinaceus showed best growth when provided with 6h light and 18h darkness which
indicated that vegetative growth of this fungus requires more of dark hours as light tends to slow down the
mycelial growth. For spawn production, wheat grain was adjudged as best substrate as it took comparatively less
number of days for full growth as compared to other grains used. Out of four substrates tested, toona sawdust
was the best substrate for cultivation of H. erinaceus as it was found good for all four strains in terms of
biological efficiency. Among 4 strains, minimum number of days for completion of spawn run was observed in
HE-18-01 in wheat straw. However, the maximum cropping period (99.40 days) was observed in HE-18-01 and
maximum biological efficiency was observed in HE-18-08 (79.80 %) for toona sawdust. Four extracts of H.
erinaceus were tested against Escherichia coli and Ralstonia solanacearum. Out of four extracts used, water
extract showed the maximum inhibition of 25 mm against both the pathogens. Ethanol and methanol extracts
also showed inhibition against R. solanacearum but methanol extract does not showed any inhibition against
E. coli. Ethyl acetate does not show any inhibition against both the pathogens tested. According to this study, it
was inferred from the results that all the extracts prepared showed significant antioxidant activity which was
tested by DPPH method. The maximum antioxidant activity was observed from methanol extract (99.20%) at
500 µg/ml concentration. So, it is clear from the study that H. erinaceus can be used as a natural antioxidant
source and is helpful for human health.