MAXIMIZATION OF IN VITRO BULBLET PRODUCTION IN LILIUM HYBRIDS
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Date
2020-10
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NAUNI,UHF
Abstract
ABSTRACT
The present investigation, “Maximization of in vitro bulblet production in Lilium
hybrids” was carried out at the Plant Tissue Culture Laboratory of Department of
Floriculture and Landscape Architecture, Dr. YS Parmar University of Horticulture and
Forestry, Nauni, Solan (HP). Three Lilium hybrid i.e. ‘Tressor’, ‘Nashville’ and ‘Acapulco’
were selected for the studies. The maximum number of uncontaminated growing culture were
observed when bulb scale was sterilized with NaOCl 3% for 30 min in all the Lilium hybrids
and for the sterilization of the leaves explants the maximum number of uncontaminated
growing culture were observed when sterilized with 3% NaOCl for 20 min was found to be
the best. For culture establishment, MS medium supplemented with BA 1.5 mg/l + NAA
0.5mg/l was found best for earliest culture establishment and resulted in maximum number of
bulblets/culture in all the three Lilium hybrids. For maximization of bulblet production, the
best medium for earliest bulblet proliferation and generating maximum number of
bulblets/culture was found to be MS medium containing BA 2.0mg/l and NAA 0.5mg/l. For
increasing the size and weight of bulblets, MS medium was supplemented with BA 1.5mg/l,
NAA 0.50 mg/l and added with 80g/l sucrose proved to be the most effective.