ASSESSMENT ON THE IMPACT OF BANANA TISSUE CULTURE PLANTLETS ON MAJOR VIRAL DISEASES
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Date
2019-11-11
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UNIVERSITY OF AGRICULTURAL SCIENCES, GKVK BENGALURU
Abstract
Tissue culture is a widely adopted technology for the propagation of virus-free
banana plants. Survey for assessment of major viruses viz., BBTV, BSV, CMV and
BBrMV in banana growing regions in southern Karnataka with tissue culture and sucker
propagated banana gardens revealed the highest mean per cent incidence of BBTV (3.41%)
followed by BBrMV (0.19%). The mean incidence of virus diseases was less (0.66%) in
tissue culture gardens compared to gardens grown using suckers (2.65%). The mean
incidence of virus disease was less (1.04%) in gardens grown using the main crop
compared to ratoon established gardens (2.13%). Colorimetric detection for banana major
viruses were standardized using nucleic acid fluorescent dyes viz., SYBR Green, Thiazole
orange and VeriPCR to the PCR amplified product of BBTV, BSV, CMV and BBrMV
infected samples produced bright green in case of SYBR green and VeriPCR whereas
bright orange in case of thiazole orange. HNB dye changed the colour from pink to violet
in infected samples of BBTV, BSV, CMV and BBrMV when applied before amplification.
Standardization of HNB dye concentration for detection of BBTV indicated that 3mM @
2µL, 5mM @ 2µL, 8mM @ 1µL and 2µL produced distinct violet with PCR product from
infected samples and pink with healthy samples. HNB dye of 3mM at the rate of 2 µL was
suitable for the detection of BBTV infected samples. The colorimetric detection methods
in the current study are cost-effective and quick as they do not require the additional steps
of electrophoresis and gel documentation