EXPLORING AND DISSECTING MOLECULAR GENETICS OF MICRONUTRIENT ACQUISITION IN VARIOUS GENOTYPES OF RICE (Oryza sativa L.) UNDER CONTRASTING WATER REGIMES
Loading...
Files
Date
2018-09-28
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Abstract
The present study was aimed at apprehending the information regarding
micronutrient acquisition by various genotypes of rice under contrasting water regimes.
Ten genotypes, which are of diverse ecotype were studied for variation in zinc and iron
content in various parts of the plant under aerobic and wetland condition. Analysis of
variance was significant for all the traits. Genetic parameters (GCV, PCV, h2, and GAM)
were recorded higher for all the parameters in both the condition. Zinc and iron
concentration of different parts of plants showed a significant difference between aerobic
and wetland condition. Grain zinc concentration was recorded significantly higher in
aerobic condition while grain iron concentration was recorded nonsignificant for most of
the genotypes. Candidate gene-specific markers, OsZIP2R and OsYSL4e showed
significant association with shoot zinc and iron content. Sequencing of monomorphic
marker OsYSL16 recorded a significant association between nucleotide variation and zinc
and iron content at different parts of rice. Differential expression of transporter genes was
recorded in various parts of contrasting genotypes (AM-65, Black rice, Jeergasamba and
Burma black) for iron and zinc content in both the condition. Quantitative Real-time PCR
study of OsIRT1 and ZIP8 in AM-65 and Jeergasamba recorded higher expression in
aerobic and wetland condition respectively suggesting that their expression is regulated
under zinc and iron deficient condition. This study revealed that cultivation system has a
major effect on zinc and iron accumulation and it mostly occurred due to differential
expression of transporter genes in different parts of rice under both the condition.