EVALUATION, MOLECULAR MARKER PROFILING AND DNA BARCODING OF ANNONA CULTIVARS GROWN UNDER NORTHERN DRY ZONE OF KARNATAKA
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Date
2019-07
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University of Horticultural Sciences, Bagalkot
Abstract
The present investigation entitled “Evaluation, molecular marker profiling and
DNA barcoding of Annona cultivars grown under Northern dry zone of Karnataka’’
was carried out during 2018-2019 using Randomized Complete Block Design with
three replications. A total of 12 Annona cultivars from the existing fruit orchard were
selected for the present study and subjected to morphological, biochemical and
molecular characterization. The treatments comprised of 12 popular cultivars, among
them 7 cultivars of Annona squamosa, one hybrid (Atemoya x Balanagar) and one
species Annona reticulata .
Variability studies demonstrated higher GCV, PCV, heritability and GAM
implying the existence of sufficient variation among the studied genotypes. High
heritability with high genetic advance was recorded for most of the traits like fruit
length, fruit diameter, pulp weight and titrable acidity. Correlation studies revealed
significant positive correlation between fruit weight and fruit diameter, no. of
seeds.Total sugars was positively correlated to reducing and non-reducing sugars.
Mahalanobis D2 analysis grouped 12 Annona cultivars into 4 clusters and high
genetic divergence was found for cultivars falling in Cluster III,II and IV. Based on
marker trait association, markers like LMCH-10,RAPD-9_960,RAPD_7_600
exhibited highest allelic diversity. The results revealed that SSR markers are highly
reproducible, co-dominant. From DNA barcoding analysis, it was possible to identify
allelic variants at single nucleotide level for 2 Universal markers viz., Matk and
Asq_Matk but not with the Rbcl region. The results obtained in the present
investigation illustrated existence of wide range of variations for most of the traits
among Annona cultivars, which would help Annona breeders to identity inter and
intra-specific variation among Annona species at early stage.