EVALUATION, MOLECULAR MARKER PROFILING AND DNA BARCODING OF ANNONA CULTIVARS GROWN UNDER NORTHERN DRY ZONE OF KARNATAKA

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dc.contributor.advisorCHOLIN, SARVAMANGALA
dc.contributor.authorK. H., JNAPIKA
dc.contributor.otherHIPPARAGI, KULAPATI
dc.contributor.otherGOLLAGI, S.G.
dc.contributor.otherREDDI, SANJEEVARADDI
dc.contributor.otherPADASHETTI, BASAVARAJ
dc.date.accessioned2020-09-23T06:45:46Z
dc.date.available2020-09-23T06:45:46Z
dc.date.issued2019-07
dc.description.abstractThe present investigation entitled “Evaluation, molecular marker profiling and DNA barcoding of Annona cultivars grown under Northern dry zone of Karnataka’’ was carried out during 2018-2019 using Randomized Complete Block Design with three replications. A total of 12 Annona cultivars from the existing fruit orchard were selected for the present study and subjected to morphological, biochemical and molecular characterization. The treatments comprised of 12 popular cultivars, among them 7 cultivars of Annona squamosa, one hybrid (Atemoya x Balanagar) and one species Annona reticulata . Variability studies demonstrated higher GCV, PCV, heritability and GAM implying the existence of sufficient variation among the studied genotypes. High heritability with high genetic advance was recorded for most of the traits like fruit length, fruit diameter, pulp weight and titrable acidity. Correlation studies revealed significant positive correlation between fruit weight and fruit diameter, no. of seeds.Total sugars was positively correlated to reducing and non-reducing sugars. Mahalanobis D2 analysis grouped 12 Annona cultivars into 4 clusters and high genetic divergence was found for cultivars falling in Cluster III,II and IV. Based on marker trait association, markers like LMCH-10,RAPD-9_960,RAPD_7_600 exhibited highest allelic diversity. The results revealed that SSR markers are highly reproducible, co-dominant. From DNA barcoding analysis, it was possible to identify allelic variants at single nucleotide level for 2 Universal markers viz., Matk and Asq_Matk but not with the Rbcl region. The results obtained in the present investigation illustrated existence of wide range of variations for most of the traits among Annona cultivars, which would help Annona breeders to identity inter and intra-specific variation among Annona species at early stage.en_US
dc.identifier.issnUHS17PGM893
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810151823
dc.keywordsANNONA, T586en_US
dc.language.isoEnglishen_US
dc.pages157en_US
dc.publisherUniversity of Horticultural Sciences, Bagalkoten_US
dc.research.problemThesisen_US
dc.subFruit Scienceen_US
dc.themeEVALUATION, MOLECULAR MARKER PROFILING AND DNA BARCODING OF ANNONA CULTIVARS GROWN UNDER NORTHERN DRY ZONE OF KARNATAKAen_US
dc.these.typeM.Scen_US
dc.titleEVALUATION, MOLECULAR MARKER PROFILING AND DNA BARCODING OF ANNONA CULTIVARS GROWN UNDER NORTHERN DRY ZONE OF KARNATAKAen_US
dc.typeThesisen_US
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