PCR BASED DETECTION OF COAGULASE GENE ENCODING STAPHYLOCOCCUS AUREUS ISOLATED FROM SUBCLINICAL MASTITIS CASES IN BUFFALOES
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Date
2010
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Abstract
The present study was carried out to characterize Staphylococcus aureus (S. aureus) isolated from mastitic
cases in buffalo based on coagulase gene. A total of 18 strains of S. aureus were isolated during processing
of 81 milk samples from 21 buffaloes. The S. aureus strains were characterized phenotypically, and were
further characterized genotypically by polymerase chain reaction using oligonucleotide primers that amplified
genes encoding coagulase (Coa).
Amplification of the Coa gene yielded a product size of 730bp in all 15 coagulase positive isolates. The
phenotypic and genotypic findings of the present strategies might provide an understanding of the distribution
of the cogulase positive S. aureus clones among buffaloes mastitis isolates, and might aid in the development
of steps to control S. aureus infections in buffaloes.
Description
TNV_IJFV_2010_5(3)53-54
Keywords
Veterinary Science