GENETIC FIDELITY STUDIES ON IN VITRO PROPAGATED PLUM (Prunus salicina L.) CVS. SANTA ROSA AND FRONTIER
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Date
2020-09
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UHF,NAUNI
Abstract
ABSTRACT
The present studies were undertaken to establish clonal fidelity in Plum cvs Santa Rosa
and Frontier by taking six year old axenic in vitro cultures and two year old tissue culture raised
plants growing in the field as experimental material. Genomic DNA was isolated from fresh
leaves of in vitro shoot cultures and plants of Plum cvs. Santa Rosa and Frontier. RAPD, ISSR
and SCoT markers were used to amplify DNA of Plum cultivars. Out of 28 RAPD primers used,
18 and 15 primers were able to amplify the genomic DNA of Plum cv. Santa Rosa and Frontier,
with the monomorphism of 96.55% and 100%, respectively and a similarity coefficient of 0.84
between both the cultivars. All the 15 ISSR primers were able to amplify the genomic DNA of
both the Plum cultivars and produced a monomorphism of 92.72% and 95.45% in Santa Rosa and
Frontier respectively. A homology of approximately 65% homology between both the cultivars.
Out of 26 SCoT primers, only 10 SCoT primers amplified the genomic DNA of Plum cvs. Santa
Rosa and Frontier resulting in monomorphism of 96.77 and 90% in both the cultivars,
respectively. On comparing Plum cvs. Santa Rosa and Frontier, 84-97% similarity was observed
between the two. All the three molecular markers were able to amplify the genomic DNA of Plum
cultivars under study with reproducible results. From the above studies it can be deduced that
Plum cvs. Santa Rosa and Frontier have a common lineage due to which large amount of
similarity can be seen in both of them at the molecular level. In vitro propagation can be
successfully used for clonal multiplication of Plum cvs. Santa Rosa and Frontier without any
undesireable variations, as validated using RAPD, ISSR and SCoT primers