FORMULATION AND DIETARY & BIOREMEDIATORY CHARACTERISTICS OF SEAWEED BASED SILAGE AND ITS UTILIZATION IN SHRIMP LARVAL REARING
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Date
2007
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Fisheries College and Research Institute, Thoothukudi, Tamil Nadu Dr. J. Jayalalithaa Fisheries University
Abstract
A fermented seaweed based marine single cell detritus (MSCD) or marine silage, a product derived from fermented green seaweed, Ulva reticulata through microbial and enzymatic digestion was tested for its dietary and bioremediatry properties in this study for the larval stages of Penaeus monodon. The enzyme cellulase was used at the concentration of 7.5ml / 100ml of base material. The microbial fermentation was carried out by using two microbes viz. lactic acid bacteria, Lactobacillus plantarum and yeast, Saccharomyces cerevisiae. They were inoculated to the enzyme treated base material at a rate of 104 cfu / ml of base material. The whole process of fermentation was carried out in a fermentor of 3.1 L capacity. The microbial propagation in the MSCD during fermentation process in the bioreactor showed a steady increase in the level of microbes i.e. „LAB‟ and yeast. The crude protein level of MSCD was estimated to be 12.50% which was inadequate to feed the shrimp larvae and hence it was enriched with an encapsulated microbound diet to a crude protein level of 32.5%. Three trails were carried out to test the dietary and bioremediatory properties of the MSCD formulated. In trial1, PL-15 showed an over all less growth and survival rate due to the low protein level of MSCD used (without enrichment). In trial 2, PL-29 was fed with MSCD (without enrichment) as a supplement along with artificial feed (commercial). The trial showed a better performance at 2.5ml of MSCD + 5% of artificial feed which was due to the presence of „LAB” in MSCD that might have increased the feed efficiency. In trial 3, the biogrowth parameters at 3-5 x 103 particles / ml / day and 3-5 x 104 particles / ml / day treatments recorded significant increment (P<0.01). The bioremediatory aspect of MSCD studied through ammonia flux showed that all the tanks fed with the eMSCD recorded significantly less ammonia level than the control (P<0.01). The yeast count in experimental tanks was inversely proporitional to the ammonia flux, which proved that there exist bioremediatory effect in MSCD