EVALUATING THE EFFICACY OF ENCAPSULATION TECHNIQUE FOR CRYOPRESERVATION OF PREIMPLANTATION BUFFALO EMBRYOS
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Date
2019-12
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Abstract
The present study was envisaged to evaluate the feasibility of the encapsulation technique for
cryopreservation of buffalo embryos. Being a biodegradable polymer, sodium alginate was chosen for
encapsulation. Different concentrations of sodium alginate – 0.5%, 1%, 2%, 3% and 4% were used for preparation
of beads in combination with 25, 50, 75 and 100 mM of calcium chloride. The optimal combination for bead
stability was found to be 1% sodium alginate with 100 mM of calcium chloride. Slaughter house derived
buffalo ovaries were subjected to in vitro maturation followed by in vitro fertilization. Two celled embryos
thus produced were divided into two groups at random. Group I embryos were cryopreserved by vitrification
after encapsulation in alginate beads whereas Group II embryos were vitrified without encapsulation. Vitrified
embryos were thawed after 7 days, retrieved from the beads and stained using Fluorescein diacetate. The
viability rate of alginate encapsulated embryos was found to be higher (83.27±0.64%) when compared to
the control group (77.34±0.44%). The survivability of thawed embryos was further evaluated by culturing
them up to morula stage. The yield of morula from the alginate encapsulated group was better than that of
the control group. Thus alginate encapsulation could be considered as an ideal approach for improving the
survival rate of cryopreserved buffalo embryos.
Description
TNV_35thVCRI_CA_Dec-2019_RBTO-7
Keywords
Veterinary Science