EVALUATING THE EFFICACY OF ENCAPSULATION TECHNIQUE FOR CRYOPRESERVATION OF PREIMPLANTATION BUFFALO EMBRYOS

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dc.contributor.authorBrindha, K
dc.contributor.authorHamini, C
dc.contributor.authorJenitta, E, et al.
dc.contributor.authorTANUVAS
dc.date.accessioned2020-03-02T05:47:09Z
dc.date.available2020-03-02T05:47:09Z
dc.date.issued2019-12
dc.descriptionTNV_35thVCRI_CA_Dec-2019_RBTO-7en_US
dc.description.abstractThe present study was envisaged to evaluate the feasibility of the encapsulation technique for cryopreservation of buffalo embryos. Being a biodegradable polymer, sodium alginate was chosen for encapsulation. Different concentrations of sodium alginate – 0.5%, 1%, 2%, 3% and 4% were used for preparation of beads in combination with 25, 50, 75 and 100 mM of calcium chloride. The optimal combination for bead stability was found to be 1% sodium alginate with 100 mM of calcium chloride. Slaughter house derived buffalo ovaries were subjected to in vitro maturation followed by in vitro fertilization. Two celled embryos thus produced were divided into two groups at random. Group I embryos were cryopreserved by vitrification after encapsulation in alginate beads whereas Group II embryos were vitrified without encapsulation. Vitrified embryos were thawed after 7 days, retrieved from the beads and stained using Fluorescein diacetate. The viability rate of alginate encapsulated embryos was found to be higher (83.27±0.64%) when compared to the control group (77.34±0.44%). The survivability of thawed embryos was further evaluated by culturing them up to morula stage. The yield of morula from the alginate encapsulated group was better than that of the control group. Thus alginate encapsulation could be considered as an ideal approach for improving the survival rate of cryopreserved buffalo embryos.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810144017
dc.language.isoenen_US
dc.pages1en_US
dc.subjectVeterinary Scienceen_US
dc.titleEVALUATING THE EFFICACY OF ENCAPSULATION TECHNIQUE FOR CRYOPRESERVATION OF PREIMPLANTATION BUFFALO EMBRYOSen_US
dc.title.alternativeInternational Symposium on Global perspectives to enhance livestock fertility through modern reproductive techniques for doubling farmer's Incomeen_US
dc.typeOtheren_US
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