IMMUNE RESPONSE IN SHEEP TO IMMUNIZATION WITH DNA ENCODING THE VARIABLE REGION OF ANTI-IDIOTYPIC ANTIBODY SPECIFIC FOR HN PROTEIN OF PESTE DES PETITS RUMINANTS VIRUS
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Date
2010-07
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KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR
Abstract
The present work was undertaken to evaluate and compare the immune responses
in sheep to a recombinant DNA vaccine coding for VH chain region of anti-idiotypic
antibody against HN protein of PPR and conventional PPRV vaccine. Twenty four
seronegative sheep to PPRV antibodies were subjected to vaccination trials. Recombinant
DNA vaccine and conventional PPRV vaccine were administered on days 0 and 21.
Humoral immune response in vaccinates was evaluated by indirect ELISA and Serum
Neutralization Test (SNT). The present study revealed high antibody titres in
conventional cell culture PPRV vaccinated groups than recombinant DNA vaccinated
group. However, the serum samples were having neutralizing titer of ≥ 8 in both the
groups. Indirect ELISA results showed significant difference between the recombinant
DNA vaccinated group and control groups indicating the capability of recombinant DNA
vaccine to elicit immune response in the total absence of antigen. The preliminary study
carried out infer that recombinant vaccine against PPR can be successfully used for
protecting sheep against PPR and further overcomes the handling of PPRV for production
of conventional cell culture vaccine.
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