“Isolation and Molecular Characterization of Zinc Solubilizing Bacteria and Evaluation of Their Potential to Influence the Growth of Maize (Zea mays)”
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Date
04-09-18
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MPUAT, Udaipur
Abstract
“Isolation and Molecular Characterization of Zinc Solubilizing Bacteria and Evaluation
of Their Potential to Influence the Growth of Maize (Zea mays)”
Aradhana Sukhwal * Dr. Vimal Sharma Zinc (Zn) is one of the essential micronutrient required for optimum plant growth and
development. Zinc solubilizing bacteria (ZSB) are potential alternates for zinc fertilizers
since it convert unavailable form of Zinc to available form. A total of seventy nine ZSB
isolates were isolated from soils of Dungarpur and Pratapgarh districts of Rajasthan. Out of
79 isolates, 32 were further selected on the basis of their potential to solubilize insoluble zinc
viz., Zn3(PO4)2, ZnCO3 and ZnO. These 32 ZSB were subjected to morphological,
biochemical, physiological and molecular characterization, screening of their plant growth
promotion activities, ability of these isolates to survive in abiotic stress conditions viz., salt,
pH, drought, antibiotic and temperature stress and evaluation of their potential to influence
the growth of maize. The results revealed that all the ZSB isolates were gram negative. The
molecular diversity by Amplified Ribosomal DNA Restriction Analysis (ARDRA) was done
in thirty two ZSB strains reveled high diversity amongst them. Based on UPGMA clustering
analysis, the strains showed significant molecular diversity and the dendogram obtained
differentiated 32 strains into 02 major clusters. The Major clusters were further sub divided
into four sub clusters. Major cluster A consisted a total 27 strains and sub cluster A1
consisted of 3 strains, A2 consisted of 24 strains and cluster B consisted of 02 strains and 3
strains (ZSB20, ZSB29 and ZSB5) stood apart and were kept as independent minor groups in
the dendogram. Out of 32 strains, 26 strains were positive for phosphorus solubilization, 13
strains were positive for silica solubilization and 26 strains for pottasium solubilization. All
of them were further tested for various PGPR traits like IAA, HCN, ammonia, siderophores
and extracellular enzymes like cellulases, chitinase, lipase, glucanase, proteases and
amylases. Results showed that 14 strains showed IAA production, 29 isolates produced
ammonia, 9 strains were positive for hydrogen cyanide, 8 isolates produced siderophores, 14
strains exhibited ACC deaminase activity, 1 strain produced EPS, 19 strains exhibited
cellulases activity, 22 strains produce protease, 14 strains produce amylase, 22 strains
exhibited lipase activity, 1 strain exhibited chitinase activity and 1 strain produced glucanase.
Pot experiment was conducted with 9 promising ZSB strains having multiple PGPR activities
and higher zinc solubilization. The results were obtained from pot studies showed that both
ZSB1 and ZSB17 were the best strains among selected ZSB strains and further selected for
field studies. These strains enhanced Zn translocation toward grains and increased yield of
Maize variety P3441 by 19.01% and 17.64% respectively
Description
solation , Molecular Characterization Evaluation, Their Potential, Influence Growth, Maize
Keywords
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Citation
Sukhwal, A. and Sharma, V.