CHARACTERIZATION AND COMPARISON OF SIDEROPHORES PRODUCINGPseudomonas STRAINS IN RELATION TO GROWTH OF REPLANTED APPLE
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Date
2018-12
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UHF, NAUNI
Abstract
ABSTRACT
The ability of fluorescent Pseudomonas isolates to produce siderophores is an important trait
to overcome replant problem of apple by killing phytopathogens to some extent. Therefore, the
objective of the present study was to characterize and compare different siderophores produced by
fluorescent Pseudomonas isolates from normal and replant sites of apple orchard. Isolation and
characterization of fluorescent Pseudomonas isolates and strains from normal and replant site of apple
orchard (Shimla HP) was done. Total eighteen Pseudomonas isolates and strains were screened out
for various plant growth promoting activities like siderophore, phosphate solubilization, antifungal
activity, plant growth regulators (auxins, cytokinins and gibberellins), lytic enzymes and production
of HCN and ammonia. On the basis of best PGP activities, two isolates Rn3 and Rn 7 were
genotypically characterized by 16S rRNA gene sequencing showed 97% and 99% similarity with
Pseudomonas moraviensis and Pseudomonas fluorescens respectively. One actinomcetes isolate from
replant site at Rauni i.e. A3 was selected for genotypic characterization on the basis of PGP activities
showed 98% similarity with Streptomyces bluensis. Optimization of media, temperature, pH, time of
incubation, nitrogen sources and carbon sources were done for the production of siderophores from
Pseudomonas strains L (P. putida), M (P. fluorescens), C & G (P. aeruginosa), which were also used
for mass multiplication of bioformulations for field trials. The production, partial purification and
characterization of siderophores was done by Sephadex G-25 column chromatography. Through SDSPAGE
electrophoresis, with molecular weight of siderophores produced by L, M, C & G was
recorded 12kDa, 11 kDa, 19 kDa and 20kDa respectively. The performance of apple plants was much
better in terms of root colonization capacity, % survival, plant establishment and increase in plant
growth in terms of plant height, number of nodes, chlorophyll cont
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