CHARACTERIZATION AND COMPARISON OF SIDEROPHORES PRODUCINGPseudomonas STRAINS IN RELATION TO GROWTH OF REPLANTED APPLE

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dc.contributor.advisorKAUR, MOHINDER
dc.contributor.authorSHARMA, PARUL
dc.date.accessioned2019-02-08T09:38:39Z
dc.date.available2019-02-08T09:38:39Z
dc.date.issued2018-12
dc.description.abstractABSTRACT The ability of fluorescent Pseudomonas isolates to produce siderophores is an important trait to overcome replant problem of apple by killing phytopathogens to some extent. Therefore, the objective of the present study was to characterize and compare different siderophores produced by fluorescent Pseudomonas isolates from normal and replant sites of apple orchard. Isolation and characterization of fluorescent Pseudomonas isolates and strains from normal and replant site of apple orchard (Shimla HP) was done. Total eighteen Pseudomonas isolates and strains were screened out for various plant growth promoting activities like siderophore, phosphate solubilization, antifungal activity, plant growth regulators (auxins, cytokinins and gibberellins), lytic enzymes and production of HCN and ammonia. On the basis of best PGP activities, two isolates Rn3 and Rn 7 were genotypically characterized by 16S rRNA gene sequencing showed 97% and 99% similarity with Pseudomonas moraviensis and Pseudomonas fluorescens respectively. One actinomcetes isolate from replant site at Rauni i.e. A3 was selected for genotypic characterization on the basis of PGP activities showed 98% similarity with Streptomyces bluensis. Optimization of media, temperature, pH, time of incubation, nitrogen sources and carbon sources were done for the production of siderophores from Pseudomonas strains L (P. putida), M (P. fluorescens), C & G (P. aeruginosa), which were also used for mass multiplication of bioformulations for field trials. The production, partial purification and characterization of siderophores was done by Sephadex G-25 column chromatography. Through SDSPAGE electrophoresis, with molecular weight of siderophores produced by L, M, C & G was recorded 12kDa, 11 kDa, 19 kDa and 20kDa respectively. The performance of apple plants was much better in terms of root colonization capacity, % survival, plant establishment and increase in plant growth in terms of plant height, number of nodes, chlorophyll conten_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810094173
dc.keywordsfluorescent Pseudomonas isolates, phytopathogens, produce siderophores,en_US
dc.language.isoenen_US
dc.pages236+XVen_US
dc.publisherUHF, NAUNIen_US
dc.subBasic Sciencesen_US
dc.subjectnullen_US
dc.themefluorescent Pseudomonas isolates, phytopathogens, produce siderophores,en_US
dc.these.typePh.Den_US
dc.titleCHARACTERIZATION AND COMPARISON OF SIDEROPHORES PRODUCINGPseudomonas STRAINS IN RELATION TO GROWTH OF REPLANTED APPLEen_US
dc.typeThesisen_US
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