Development of transgenic chickpea (Cicer arietinum L.) plants with lectin receptor- like kinase (Lec-RLK) gene for improving salt tolerance)
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Date
2018
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CCSHAU
Abstract
Abiotic stresses unpropitiously affect growth and productivity and spark off a series of m
orphological, physiological, biochemical and molecular changes in plants. Drought, salinity,
high &low temperature, are the most common abiotic stresses that plants mostly face.
Transgenic chickpea plants expressing Lec-RLK gene have been generated using a novel
process of Agrobacterium-mediated genetic transformation.Multiple evaluation strategies,
such as PCR, RT-PCR and Southern blot were employed for characterization of the
integration and expression of Lec-RLK gene in chickpea variety HC-1.Tolerance under
100mM salt stress was studied by performing physio-biochemical analysis in T1 generation
chickpea plants.The Lec-RLK carrying transgenic chickpea plants showed higher tolerance to
salt stress than wild-type plants in T1 generation.Transgenic plants revealed higher levels of
proline content,chlorophyll content, MDA content, total soluble sugar and decreased %age of
membrane injury than those in wild-type chickpea plants under 100mM NaCl stress in 15
days. Results from the current study suggest that the expression of the Lec-RLK gene in
transgenic chickpea plants induces the synthesis of proline & decreased membrane injury
improves the tolerance of plants under stress.The study demonstrated potentialities of
developing salt tolerant transgenic chickpea and provides an opportunity for further test and
advancement to reach the field utility level.
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