TISSUE CULTURE STUDIES IN WHEAT [Triticum aestivum ( L.) em. Thell]
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Date
2004
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Publisher
MPUAT, Udaipur
Abstract
The present investigation entitled “Tissue Culture Studies in Wheat (Triticum aestivum
L.)” was carried out in Biotechnology Laboratory of Department of Plant Breeding and
Genetics, Rajasthan College of Agriculture, Udaipur.
The main objectives of the study were to induce callusing in bread wheat and to study the
effect of PEG induced stress on callus development of different genotypes of Triticum
aestivum L. e.g. Hindi-62, Raj-3077, Kailash, PBN-151, C-306, Raj-3765, Kalyan Sona,
K-9708, GW-190 and HD-2329 was comprised three experiment viz. (i) standardization
of protocol to induce callusing in bread wheat (ii) application of the above protocol to the
ten genotypes (iii) effect of the PEG induced stress on callus development of different
genotypes of wheat. Observations were recorded on callus growth as days to callus
induction, callus fresh and dry weight and callus moisture percentage.
In first experiment, various concentrations and combinations of phytohormones were
applied on mature embryos/seed explants of genotype Hindi-62. The treatment 10 mg/l
proved to be better in terms of earliest days to callus induction, greater callus proliferation
highest fresh and dry weight of callus.
In second experiment, treatment combination 10 mg/l 2,4-D was applied on ten genotypes
differences among the genotypes were found to be significant for days to callusing, callus
fresh weight, callus dry weight and callus moisture percentage. Genotype Hindi-62
responded earliest callusing maximum callus fresh weight, callus dry weight and callus
moisture percentage.
In third experiment selected protocol with 0% PEG, 10% PEG and 20% PEG was applied
on above ten genotypes to study the effect of PEG-6000 induced stress on callus growth.
Significant differences were present between the genotypes, PEG concentrations and their
interactions for callus fresh weight, dry weight and moisture percentage.
In genotypes Raj-3077, Raj-3765, Kalyan Sona and Hindi-62 per cent decrease in callus
fresh weight was found to be less than other genotypes revealing their high tolerance to
PEG induced stress. Generally, there was decline in fresh weight in all the genotypes at
10% PEG-6000. Whereas at 20% PEG-6000 no callusing was reported in any of the ten
genotypes taken in the present study.
Description
TISSUE CULTURE STUDIES IN WHEAT
[Triticum aestivum ( L.) em. Thell]
Keywords
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Citation
Dhayal and Shah, 2004