2-D Electrophoretic profiling of Rhizobium isolates of Chickpea (Cicer arietinum L.) collected from various soil regimes
Loading...
Date
2013
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Birsa Agricultural University, Kanke, Ranchi, Jharkhand
Abstract
Out of several gases present in the atmosphere nitrogen share the major portion (about 71%)
and is found in the di-nitrogen (an inert) form. It is the component of many bio-molecules
required for the growth and development of all organisms. Most of the eukaryotes are
incapable of utilizing nitrogen directly from the environment; only a certain group of
prokaryotes are genetically feasible to fix the atmospheric nitrogen into the biologically useful
form like ammonia which is further utilized by eukaryotes. Rhizobium a gram negative
bacteria associates symbiotically with legume crop and are genetically feasible in reducing
(fixing) atmospheric nitrogen for leguminous crop. Legumes in turn provide shelter and
energy to them. The specificity of Rhizobia to inoculate legume falls either in broad range
host specificity to narrow range host specificity. Several abiotic stresses adversely influence
the activity of Rhizobium. Soil pH is one of the stresses which hamper the symbiotic
association between the two. As per the reports soil pH in the range of 6.5-7.0 are considered
best in the case of leguminous crop for the optimal activity of the bacteria. Soil pH below or
above this range minimizes the Biological Nitrogen Fixation (BNF) through Rhizobia.
Two-dimensional electrophoresis (2-D electrophoresis) is a powerful and widely used
method for the analysis of complex protein mixtures extracted from cells, tissues, or other
biological samples. This technique sorts proteins according to two independent properties in
two discrete steps: the first-dimension step, isoelectric focusing (IEF), separates proteins
according to their isoelectric points (pI); the second-dimension step, SDS-polyacrylamide gel
electrophoresis (SDS-PAGE), separates proteins according to their molecular weights. Each
spot on the resulting two-dimensional array corresponds to a single protein species in the
sample. Thousands of different proteins can thus be separated, and information such as the
protein pI, the apparent molecular weight, and the amount of each protein is obtained.
The aim of my work is to perform 2-D Electrophoretic profiling of Rhizobium isolates of
Chickpea (Cicer arietinum L.) collected from various soil regimes. Cicer arietinum plant has
been taken as a model system for the collection of nodules. Chickpea, as a legume, improves
soil fertility through (BNF) biological nitrogen fixation. Chickpea is a crop that provides cash
income from its grain. It requires no N fertilizers owing to its ability to fix atmospheric N, and
in rotation can improve the N nutrition and yield of subsequent cereals, One of the most
important factors that affect the efficiency of symbiosis between Rhizobia and plants is the pH
of the soil in which they interact. The host plant to any symbiotic Rhizobium appears to be the
Description
2-D Electrophoretic profiling of Rhizobium isolates of Chickpea (Cicer arietinum L.) collected from various soil regimes
Keywords
null